Word Finder - plasmid

definition

A loop of double-stranded DNA that is separate from and replicates independently of the chromosomes, most commonly found in bacteria, but also in archaeans and eukaryotic cells, and used in genetic engineering as a vector for gene transfer.

The different forms of the plasmid are then separated by gel electrophoresis.

Initial experiments are designed to determine if afferent lymph DC carry plasmid, make GFP and/or take up free GFP from the lymph plasma.

Here the enzyme Eco RI is used to sever plasmid DNA which is then re-joined using DNA ligase.

A population of rat hybridoma cells were transfected with an expression plasmid encoding a humanized monoclonal antibody.

The bacterium contains a section of DNA called a plasmid in addition to its usual component of DNA.

If you have a plasmid that might be interesting to sequence in this context please contact Christopher Thomas.

Then you can try and see if you can transform one plasmid and then make that strain competent and then transform the second plasmid.

The third step is to join the new gene into the bacterial plasmid.

Place the modified plasmid into a dish containing leaf disks cut from the plant to be modified.

Both pUC18 plasmid and transgenic maize DNA were used in the experiments.

No genetic maps were given on the constructs or the plasmids used, nor whether any plasmid sequences are integrated into the transgenic plants.

Gateway technology is very suitable for high-throughput production of expression plasmid of FL cDNA.

Therefore, the helper plasmid lacking one or the other of the ITS cannot integrate itself into target DNA.

The only information on the plasmid not blacked out was that it was an E coli plasmid.

Otherwise those original donor plasmid may interfere with the transformation later.

These replication initiators recognize the plasmid, begin its replication initiators recognize the plasmid, begin its replication and control the number of copies made, but ignore the host DNA.

For my 4th year project I will be working with the university genetics department to model certain aspects of plasmid replication.

The gene remained active by stimulating expression from a plasmid containing the chloramphenicol acetyl transferase gene under the control of the tat dependent promoter.

These replication initiators recognize the plasmid, begin its replication and control the number of copies made, but ignore the host DNA.

No transformants were obtained after the plasmid DNA was exposed to silage effluent or rumen fluid for longer than 1 min.

It emphasizes the importance of achieving maximal plasmid DNA purity when performing DNA transfection experiments that focus on cell survival.